Undergraduate Summer Research Program
Week Three
Microscopy, Enumeration, and Digital Imaging:
Mat Layer Processing:
Site: Fairy Red Layer
Image – Visible Light
|
10X |
40X |
|
|
|
Image – F Fluorescence
|
10X |
40X |
|
|
|
|
10X |
40X |
|
NA |
|
Image – R Fluorescence
Grid-Based Counts:
|
Replicate 1 |
Fluoresces |
|
4 filaments in grid from run-off water filter
|
|
|
|
|
10xL
20xL
20x F
40x grid
40x
light
40x R
40x FChemical Analysis of Acid-Digested Samples for Metal
Assessment
Specific Post-Acid
Digestion Results:
|
Aluminum |
0.00 mg/L |
|
Copper |
0.00 mg/L |
|
Iron |
0.18 mg/L |
|
Zinc |
0.04 mg/L |
|
No manganese |
NA |
Pigment Analysis
Methanol Extraction:
|
Methanol Extraction
Procedure: Methanol extraction method
is useful for characterization at the phylum-level. Methanol extraction method only removes the
naked pigment. |
Peaks on Graph |
|
|
Methanol Extraction
Results:
|
Wavelength (nm) |
ABS |
|
470 .0 |
2.040 |
|
|
604.0 |
0.149 |
|
|
667.0 |
0.136 |
|
|
769.0 |
0.293 |
|
|
976.0 |
0.009 |
|
In vivo Bead-Beating:
|
In vivo Bead-Beating
Procedure: In vivo extraction removes
the pigment with the protein it’s bound to.
It’s useful for characterization up to the genus-level. |
Peaks on Graph |
|
|
In vivo Bead-Beating Results:
|
Wavelength (nm) |
ABS |
|
461.0 |
0.547 |
|
|
750.0 |
0.075 |
|
|
795.0 |
0.109 |
|
|
890.0 |
0.156 |
|
DISCUSSION:
Green sulfur bacteria presence is indicated by peaks at 750nm in the in vivo, and 667nm in the methanol.
Purple/ proteobacteria presence is
indicated by peaks at 795nm and 890 nm in the in vivo, and 769nm in the
methanol.